Indian researchers have developed a PCR-RFLP method to authenticate 4 shrimp species of the family Penaeidae, namely, Litopenaeus vannamei, Penaeus monodon, P. semisulcatus and Fenneropenaeus indicus. PCR amplification was performed targeting 16S rRNA/tRNAval region having an amplicon size of 530 bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3. Subsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded a distinct RFLP pattern for each species of shrimps having fragment sizes below 150 bp. The method works with processed shrimp products, and was validated with commercial products.
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