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Indian researchers have developed a PCR-RFLP method to authenticate 4 shrimp species of the family Penaeidae, namely, Litopenaeus vannamei, Penaeus monodon, P. semisulcatus and Fenneropenaeus indicus.  PCR amplification was performed targeting 16S rRNA/tRNAval region having an amplicon size of 530 bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3. Subsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded a distinct RFLP pattern for each species of shrimps having fragment sizes below 150 bp. The method works with processed shrimp products, and was validated with  commercial products.

Read the abstract here

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