German and Italian researchers have used a proteomics based assay to distinguish wild from farmed salmon. A total of 13 farmed and 13 wild Canadian salmon (Salmo salar) species were extracted and digested with trypsin. The peptide digest was analysed by an optimised LC- MS system(quadrupole time-of-flight mass spectrometer) followed by statistical analysis based on principal component (PC) analysis.This untargeted approach, using a data-independent acquisition MS scheme, demonstrated the ability to effectively discriminate salmon belonging to the two classes. Furthermore, selected peptides showing high loadings on PC1 could represent potential targeted candidate peptide markers able to discriminate farmed from wild-type salmon.
Coffee is currently the second largest commodity on the world market. Brazilian researchers have written a comprehensive review on the development and use of chromatograpy from paper to gas and hplc, and finally ultra-performance liquid chromatography coupled with tandem mass spectrometry to confirm adulteration and fraud in coffee.
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