A Real-Time PCR Method to Authenticate Cuttlefish

4167177917?profile=RESIZE_710xThe common cuttlefish (Sepia officinalis) is highly appreciated by consumers, and Spanish reseachers have developed a rapid assay to determine its authenticity. The method  is based on TaqMan real-time PCR (Polymerase Chain Reaction) method to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify. No cross-reactivity was detected with any other species. The assay differentiates S. officinalis from other species of the genus Sepia and other cephalopod species, and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. 

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