Locate methods for authenticity testing

The tabs below include lists of test methods, or links, to assist location of test methods for food authenticity.

The following list includes Standard Operating Proceures prepared in the course of FSA and DEFRA Food Authenticity projects. The list is currently for information only; links or available documents will be added as available. In the mean time, SOPs or other procedures can be found by searching for project number or SOP title on the FSA or DEFRA websites.

Note: Appearance on this list does not constitute approval for a particular purpose and no warranty is given relating to the performance in practice of the SOPs listed here.

STANDARD OPERATING PROCEDURES (SOPs) PREPARED UNDER THE FOOD AUTHENTICITY PROGRAMME.

SOP Related to Project Number Title Author (Organisation) Web Link/Document
Q01055 SOP for the Extraction of Desmin from Meat Samples Cheryl Wells (NTU) 2006 SOP: Extraction of Desmin
Q01055 SOP for the Analysis of Desmin Enriched Meat Extracts Cheryl Wells (NTU) 2006 SOP: Analysis of Desmin
Q01066 SOP for the Determination of the Geographical Origin of Uncooked Beef Meat.(Determination of d13C‰ and d15 N‰) Simon Kelly (IFR) 2006 http://sciencesearch.defra.gov.uk/...
Q01081 SOP for the Determination of Corn-Fed Status of Chicken Meat using the 13C/12C Isotope Ratio of the Protein Fraction of the Meat Paul Reece (Fera) 2008 SOP: Corn fed chicken
Q01083 SOP for the Quality Control for the Analysis of Exotic meat Samples Using Real Time PCR (TaqmanTM). Hez Hird (Fera ) 2005 Appendix 4.1 http://sciencesearch.defra.gov.uk/...
Q01083 SOP for the Preparation of Reagents Required for RT-PCR Qualitative Analysis of Exotic Meat Species in Meat Products (CTAB) 2005 Appendix 4.2 http://sciencesearch.defra.gov.uk/...
Q01083 SOP for the CTAB Extraction of DNA from Meat Products for Qualitative Exotic Meat Species Identification. 2005 Appendix 4.3 http://sciencesearch.defra.gov.uk/...
Q01083 SOP for the Analysis of PCR Inhibitors in Meat Samples Using Real Time PCR (TaqmanTM) 2005 Appendix 4.4 http://sciencesearch.defra.gov.uk/...
Q01083 SOP for the Analysis of Exotic Meat Species ( Using Real Time PCR (TaqmanTM) 2005 Appendix 4.5 http://sciencesearch.defra.gov.uk/...
Q01084 SOP for the Quality Control and Data Analysis of the Qualitative Analysis of Commercial Meat Species in Meat Products Using Real Time PCR (TaqmanTM). Hez Hird (Fera ) 2005 Appendix 4.1 http://webarchive.nationalarchives.gov.uk/...
Q01084 SOP for the Preparation of Reagents for the Analysis of Commercial Meat Species in Meat Samples Using Real Time PCR (TaqmanTM) (CTAB). 2005 Appendix 4.2 http://webarchive.nationalarchives.gov.uk/...
Q01084 SOP for the CTAB Extraction of DNA from Meat Samples for Real Time PCR Identification of Commercial Meat Species (TaqmanTM) 2005 Appendix 4.3 http://webarchive.nationalarchives.gov.uk/...
Q01084 SOP for an Internal Positive Control Protocol for the Assessment of Inhibition of the Polymerase Chain Reaction (PCR)- Performed Using ‘Real Time’ PCR and an ABI 7700 Instrument. 2005 Appendix 4.4 http://webarchive.nationalarchives.gov.uk/...
Q01084 SOP for the Analysis of Commercial Meat Species (Pork, Beef, Lamb, Chicken and Turkey) Using Real Time PCR (TaqmanTM) 2005 Appendix 4.5 http://webarchive.nationalarchives.gov.uk/...
Q01086 SOP for the Determination of the Geographical Origin of Poultry Simon Kelly (IFR) 2008 http://webarchive.nationalarchives.gov.uk/...
Q01093 SOP for the Detection of Bovine and Porcine Fibrinopeptides in Raw and Cooked Meats Paul Reece (Fera) 2007 SOP: Fibrinogen
Q01095 SOP for the Extraction of DNA from Vegetable Oil Samples John Dooley (Campden BRI) 2006 http://sciencesearch.defra.gov.uk/...
Q01095 SOP for the Use of a DNA-Based Approach for the Detection of Unrefined Hazelnut Oil in Virgin Olive Oil. John Dooley (Campden BRI) 2006 http://sciencesearch.defra.gov.uk/...
Q01099 SOP for the Discrimination of Salmon Species in Canned Products by PCR-RFLP Analysis using the Agilent 2100 Bioanalyser Steve Garrett (Campden BRI) 2007 http://sciencesearch.defra.gov.uk/...
Q01099 SOP for the Discrimination of Tuna Species in Canned Products by PCR-RFLP Analysis on the Agilent 2100 Bioanalyser Steve Garrett(Campden BRI) 2007 http://sciencesearch.defra.gov.uk/...
Q01099 SOP for Discrimination of King & Queen Scallop Species by PCR-RFLP Analysis using the Agilent 2100 Bioanalyser Steve Garrett (Campden BRI) 2007 http://sciencesearch.defra.gov.uk/...
Q01101 SOP for a Microscopy Protocol for Identifying Mechanically Separated Meat (MSM) for Pork, Chicken and Turkey Kathy Groves (LFR) 2007 Appendix II http://sciencesearch.defra.gov.uk/...
Q01105 SOP for the Extraction of Meat Products (Raw and Processed) and the Analysis of Added Offal. Ellen Billett (NTU) 2012 http://sciencesearch.defra.gov.uk/...
Q01106 SOP for the Detection and Semi-Quantification of T. aestivum Addition in T. durum Pastas and Semolinas by PCR followed by Analysis using the Agilent 2100 Bioanalyser Gordon Wiseman (Premier Foods) 2007 Appendix 8.1 http://randd.defra.gov.uk/...
Q01107 SOP for the Genetic Identification of Common and Exotic Meat Species using the Agilent 2100 Bionalyser Capillary Electropheresis System with Tepnel Bead Extraction Hez Hird (Fera ) 2009 http://sciencesearch.defra.gov.uk/...
Q01107 SOP for the Genetic Identification of Common and Exotic Meat Species using the Agilent 2100 Bioanalyser Capillary Electropheresis System with CTAB Extraction Hez Hird (Fera) 2009 http://sciencesearch.defra.gov.uk/...
Q01108 SOP for the Quantitative Analysis of Adulteration of Basmati Rice with the Varieties - Sherbati, Mugad Sugandha, PAK 386 or Superfine using the Agilent 2100 Bioanalyser (SOP2-RM201) Katherine Steele (Food DNA Services 2008 FSA SOP: Basmati Quant Bioanalyser RM201
Q01108 SOP for the Quantitative Analysis of Adulteration of Basmati Rice with the Varieties - Sherbati, Mugad Sugandha, PAK 386 or Superfine using the Agilent 2100 Bioanalyser (SOP3-INDEL B8) Katherine Steele (Food DNA Services) 2008 FSA SOP: Quant Indel Bioanalyser
Q01108 SOP for the Detection of Non-Permitted Rice Varieties by INDEL-PCR Analysis using the Agilent 2100 Bioanalyser (SOP1) Katherine Steele (Food DNA Services) 2008 FSA SOP: Qual Indel Bioanalyser
Q01108 SOP for the Adulteration of Basmati Rice with Sherbati, Mugad Sugandha, Pak 386 or Superfine (Beckman Quantitative SSR) Katherine Steele (Food DNA Services) 2008 FSA: Beckman Quant (not in FSA SOP format)
Q01108 SOP for the Identification of Selected Basmati Rice Varieties (Beckman qualitative SSR) Katherine Steele (Food DNA Services) 2005 FSA: Basmati Beckman Qual (not in FSA SOP format)
Q01109 SOP for the Genetic Identification of Common African Wildmeat Species using the Agilent 2100 Bioanalyser Rob Ogden (Food DNA Services) 2009 FSA SOP: Bushmeat Agilent
Q01114 SOP for the Determination of Grapefruit Juice in Orange Juice using the Agilent 2100 Bioanalyser Angus Knight (LFR)  
Q01114 SOP for the determination of Mandarin Juice in Orange Juice using the Agilent 2100 Bioanalyser Angus Knight (LFR) 2010 FSA: Mandarin Bioanalyser
Q01118 SOP for the Detection and Speciation of Gelatine in Vegetarian Meals Helen Grundy (Fera) Appendix 1 2008 http://sciencesearch.defra.gov.uk/...
Q01122 SOP for the Determination of Theobromine and Caffeine in Cocoa and Chocolate by HPLC Alan Richards (Durham PA) 2011 Appendix 7 http://sciencesearch.defra.gov.uk/...
Q01123 SOP for the Determination of the ‘Global’ Deuterium/Hydrogen (2H/1H) and Oxygen-18/Oxygen-16 (18O/16O ) Ratio of Bulk Materials. Simon Kelly (IFR) 2008 http://sciencesearch.defra.gov.uk/...
Q01123 SOP for the Determination of the ‘Global’ or ‘Average’ Carbon-13/Carbon-12 (13C/12C) and Nitrogen-15/Nitrogen-14 (15N/14N) Ratio of Bulk Materials. Simon Kelly (IFR) 2008 http://sciencesearch.defra.gov.uk/...
Q01123 SOP for the Determination of the ‘Global’ or ‘Average’ Sulphur-34/Sulphur-32 (34S/32S) Ratio of Bulk Materials Simon Kelly (IFR) 2008 http://sciencesearch.defra.gov.uk/...
Q01123 SOP for Strontium-87 Isotope Analysis of Organic Material Simon Kelly (IFR) 2008 http://sciencesearch.defra.gov.uk/...
Q01123 SOP for Trace Element Analysis of Organic Material Simon Kelly (IFR) 2008 http://sciencesearch.defra.gov.uk/...
Q01129 The Development and Validation of DNA Marker Methods for the Verification of Meat from Wild Boar. Hez Hird (FERA)  
Q01130 SOP for the Genetic Identification of Traditional Breeds of Pig using DNA SNP Markers Rob Ogden (Gen-Probe) 2011 (Appendix IVa) http://sciencesearch.defra.gov.uk/...
Q01130 SOP for the Genetic Identification of Traditional Breeds of Cattle using DNA SNP Markers Rob Ogden (Gen-Probe) 2011 (Appendix IVb) http://sciencesearch.defra.gov.uk/...
Q01131 SOP for a Microscopy Protocol for Identifying Mechanically Separated Meat (MSM) for Beef and Lamb. Kathy Groves (LFR) 2011 Appendix II http://sciencesearch.defra.gov.uk/...
Q01132 SOP for the Micro-Method for the Determination of Hydroxyproline in Gelatine Extracts Helen Grundy (FERA)  
Q01132 SOP for the Isolation of Gelatine from Chicken Fillet Preparations Helen Grundy (FERA)  
Q01132 SOP for the LC/MS/MS of Tryptic Digests of Chicken Exudate Extracts to Determine the Species Origin of Gelatine Helen Grundy (FERA)  
Q01132 SOP for the Tryptic Digestion of Gelatine Helen Grundy (FERA)  
FA0106 SOP for the Extraction of a Heart Marker Protein in Raw and Processed Meat Products and Its Subsequent Detection and Quantification by an ELISA Assay Ellen Billet (NTU) SOP For heart marker protein extraction
FA0114 SOP for the Detection of Previously Frozen Poultrymeat by Determination of HADH Activity LGC 2013 Appendix 1 http://randd.defra.gov.uk/Default.asp...
FA0116 Genetic Identification of Fish Species using DNA Barcoding Labelfish consortium (2014) FoodAuthenticity Documents site
FA0117 SOP for Development of a Two-Step Method to Determine Vegetable Oil Species in Vegetable Oil Mixtures Tassos Kaodis (University of Belfast) 2013 http://sciencesearch.defra.gov.uk/...
FA0118 SOP for the Genetic Identifications of Cod, Hake and Sole Populations using DNA SNP Markers Rob Ogden (Trace Wildlife Forensics) 2014 Appendix III Download from FoodAuthenticity Documents site
FA0122 SOP for the extraction of offal marker proteins in raw and processed meat products, and their subsequent detection and semi-quantification Lyndsey Durose, Nottingham Trent University, 2016

Download SOP from FoodAuthenticity Documents site

Link to research report

FA0125 SOP for the Genetic Identification of Traditional Cow Breeds using DNA SNP Markers Rob Ogden (Trace Wildlfe Forensics) 2012 https://documents.foodauthenticity.uk/...
FA0125 SOP for the Genetic Identifications of Traditional Pig Breeds using DNA SNP Markers Rob Ogden (Trace Wildlife Forensics ) 2012 http://sciencesearch.defra.gov.uk/...
FA0137 SOP for the Environmental Monitoring of Meat Processing Plants LGC

Link to research report

FA0146 Draft standard operating procedure for the quantitation of horse dna relative to mammalian dna in raw meat samples LGC, Teddington

Download SOP from FoodAuthenticity document site

Link to research report

FA0158 Development and validation of the proposed methodology to verify vegetable oil species in mixtures of oil Queen's University, Belfast

Download SOP from FoodAuthenticity document site

Link to research report