A real-time PCR-based screening assay was developed for the detection of crustaceans in food. In order to cover most relevant species in one analytical step, PCR systems were newly developed for the detection of prawns (Penaeidae), lobster (Homarus sp.), Common shrimp (Crangon crangon), river prawns (Macrobrachium sp.) and Chinese mitten crab (Eriocheir sinensis). In addition, a published system targeting Northern prawn (Pandalus borealis) was selected. All PCR-systems are based on mitochondrial 16S rRNA gene sequences, and were optimised to be run with a standard programme at a universal annealing temperature of 60 °C. After validation, the assay was tested on a range of food products. The assay enables detection of multiple species of market relevance.
Read the abstract at: RT-PCR Detection of Crustaceans