This paper describes a method based on combining a streamlined DNA extraction method and a SYBR Green quantitative polymerase chain reaction (SyG-qPCR) assay to generate a ready-to-use kit for rapid detection of pork admixtures in processed meat products. The method utilises a rapid and efficient DNA extraction from samples and PCR analysis, which were completed in 10 minutes. The qPCR assay utilised repetitive LINE-1 elements specific to the genome of Sus scrofa domesticus (pig) as a target and incorporated internal controls. The method was validated using 121 processed meat products, and amplification was consistently detected only in samples containing pork.
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