Indian researchers have developed a PCR method based using 3 different primers on the mitochondrial gene 12S rRNA. The method produces a cow specific amplicon (346bp) and a buffalo specific amplicon (220bp). The method can detect 0.5% addition of buffalo milk to cow milk. Although this test has been used in India to determine buffalo milk adulteration of cow milk, it could be adapted to determine cow milk adulteration of buffalo milk e.g. in the case of mozzarella cheese.
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