quantitative meat species (2)

Danish researchers have published a review of the wide range of analytical methods, which aim to quantify meat species in meat products and their limits of detection (LOD). The review attempts to address in particular, the problems associated with a correlation from quantitative DNA based results to meat content (w/w). The aim is to make researchers aware of the problems of expressing DNA results as meat content (w/w) in order to find better alternatives. 

1337352593?profile=RESIZE_710x Read the full paper

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The aim of this project was to evaluate the applicability of selected modern molecular biology methods to reliably detect and quantify meat species around the 1% (w/w) level for enforcement action with a focus on processed meat products. Three techniques were evaluated, which had quantitative potential for trace ingredient detection: real-time PCR (qPCR), droplet digital PCR (ddPCR), and a label-free mass-spectrometry (MS) approach.

Results of the research indicated that both qPCR and ddPCR demonstrated good qualitative and quantitative analytical performance at the 1% (w/w) adulteration level for enforcement action (with an associated 3-27% coefficient of variation). Across all adulterant levels investigated, ddPCR generally showed tighter precision estimates, particularly at the 0.1% (w/w) levels and with the highly processed canned meat sample. The label-free MS technique demonstrated clear qualitative capability, but did not demonstrate comparable accuracy for quantitative determinations.  

  Read the full FA0157 report.

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