Greek researchers have evaluated using DNA markers SNPs (single nucleotide polymorphism) and SSRs (simple sequence repeats) combined with high resolution melting (HRM) to authenticate monvarietal olive oils and the quantitative determination of adulteration with other plant oils (maize and sunflower oils). The SSR-HRM was more efficient in distinguishing monovarietal olive oils, while the SNP-HRM assay was more reliable in discriminating olive oil blends. HRM was also used for the detection of adulteration of olive oil with oils of different plant origin by using plastid trnL indels and SNPs. The trnL-indels-HRM showed higher discrimination power than the trnL-SNP-HRM in determining adulteration in olive oil.
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