German researchers have developed and undertaken an in-house validation of an LC-MS and LC-MS/MS-based assay for authenticity testing of certain fish species. An enzyme digest and trypsin hydrolysis of freeze dried samples of commercially available Lutjanus malabaricus (red snapper) and Sebastes spp. (redfish) were analysed by LC-electrospray-MS and MS/MS assay with multivariate analysis, which enabled the two species to be distinguished from each other. An additional 68 samples [nine additional marine species such as pangasius (Pangasianodon hypophthalmus), salmon (Salmo salar), turbot (Scophthalmus maximus), plaice (Pleuronectes platessa), sole (Solea solea), lemon sole (Glyptocephalus cynoglossus), halibut (Reinhardtius hypoglossoides), red salmon (Oncorhynchus nerka), and great scallop (Pecten jacobaeus)] served as blinded negative controls to ensure the specificity of the assay.
In this article, untargeted methods capable determining the authenticity of foods are reviewed. The article also reviews and discusses a more specific focus on methods for detecting fish adulteration/substitution and involving sensory, physicochemical, DNA-based, chromatographic and spectroscopic measurements, combined with chemometric tools.
Read the full review at: untargeted methods for fish
JRC (the EU Commission's Joint Research Centre) has just published a report summarising 20 years of EU funded projects on the development of emerging technologies to identify fish species and improve fish trade traceability. The report covers methods based on DNA amplification, DNA sequencing, DNA arrays, proteomics and chemical profiling.
Read the report at: JRC fish species report
This paper records the outcome of the latest and largest multi-species, transnational survey of fish labelling to date, which demonstrates an apparent reduction of seafood mislabelling in Europe. The authors argue that recent efforts in legislation, governance, and outreach have had a positive impact on industry regulation coupled with successful molecular biology methodology.
Read more at: http://usir.salford.ac.uk/37241/1/Mariani%20et%20al_Final-FEE-Dec2015.pdf
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