digital pcr (3)

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Ancient wheat varieties - einkorn, emmer and spelt are called "hulled wheat" because the hulls are quite tough and not removed during threshing, unlike common and durum wheat, where the hulls are more brittle, and are termed "hulless wheat". Ancient wheat varieties are increasing in popularity, especially with organic farmers, because of their organoleptic qualities. They are collectively called the Italian term "farro". There is a siginificant price differential between hulled and hulless wheat, and hence a requirement to verify labelling of products containing einkorn, emmer and spelt. In this study, researchers have developed a digital PCR method that will quantify the amount of "farro" and hulless wheat (common or durum wheat) in flours and wheat based products. It was tested between two laboratories with a range of products, and apart from two products (which may have been labelling incorrectly), the two laboratories determination matched each other and the labelling very closely. There was no or very little cross reactivity with barley, oat and rice.

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Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications (including for food authenticity), and our understanding of its technological potential.

Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required.

To support the community and reflect the growing use of dPCR, an update to dMIQE, dMIQE2020, has been published including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process.

Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

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The dMIQE2020 guidelines have been added to a new 'Guidelines' area of the Quality section of the Food Authenticity Network.

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