pcr-elona - News - FoodAuthenticity2024-03-28T23:14:58Zhttps://www.foodauthenticity.global/blog/feed/tag/pcr-elonaNew Duplex PCR Assay to Detect Pork in Beef and Chicken Productshttps://www.foodauthenticity.global/blog/new-duplex-pcr-assay-to-detect-pork-in-beef-and-chicken-products2019-03-09T12:10:14.000Z2019-03-09T12:10:14.000ZMark Woolfehttps://www.foodauthenticity.global/members/MarkWoolfe<div><p>Researchers have reported the development of a sensitive and reliable method of pork adulteration in beef and chicken products based on PCR–Enzyme Linked Oligonucleotide Assay (ELONA). The asssay uses species-specific tailed primers for duplex amplification, and simple dilution of the PCR reactions for direct colorimetric detection <em>via </em>hybridisation, eliminating the need for any other post-amplification steps. It was validated using DNA add-mixtures as well as DNA extracted from raw meat mixtures, and 0.5–1% <em>w/w</em> pork could be easily detected when mixed with beef or chicken. </p>
<p><a href="{{#staticFileLink}}1337352593,RESIZE_710x{{/staticFileLink}}" target="_blank" rel="noopener"><img class="align-left" src="{{#staticFileLink}}1337352593,RESIZE_710x{{/staticFileLink}}" width="350" /></a> Read the abstract <a href="http://www.sciencedirect.com/science/article/pii/S0308814619304182" target="_blank" rel="noopener">here</a></p>
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