isothermal nucleic acid amplification - News - FoodAuthenticity2024-03-28T15:11:06Zhttps://www.foodauthenticity.global/blog/feed/tag/isothermal%2Bnucleic%2Bacid%2BamplificationA Simple Isothermal Nucleic Acid Amplification Method for the Possible On-Site Identification for Adulteration of Mutton with Porkhttps://www.foodauthenticity.global/blog/a-simple-isothermal-nucleic-acid-amplification-method-for-the-pos2018-12-03T20:14:19.000Z2018-12-03T20:14:19.000ZMark Woolfehttps://www.foodauthenticity.global/members/MarkWoolfe<div><p>Conventional nucleic acid amplification methods are reliable, but the requirement of complex equipment, skilled technicians and long operation time limit their on-site use. Here, a simple denaturation bubble-mediated strand exchange amplification method (SEA) requiring only a pair of primers and one polymerase was first reported for identifying adulteration of pork source by targeting the species-specific mitochondrial DNA sequence. The SEA method could detect 1% pork total DNA by both colorimetric and fluorescence determination. The whole detection process could be finished within 1 hour by coupling with fast tissue DNA extraction method, only requiring a simple heating block, and hence making it suitable for on-site meat species identification. </p>
<p>Read the abstract <a href="http://www.sciencedirect.com/science/article/pii/S0956713518305851" target="_blank" rel="noopener">here</a></p>
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